Analysis of DNA damage is key for cancer research. High-content screening allows testing the effect of different conditions on genotoxicity in an efficient manner.
After acquiring the data, as a first step, the DAPI nuclei are segmented. Intensity measurements of another nuclear signal (red signal) determines the cell cycle stage. It is then possible to classify these nuclei based on intracellular DNA-damage foci (EdU, green signal) using a parent-child operation in the analysis pipeline.
The solution allows for fast and flexible stratification of nuclei according to a diverse array of parameters for an in-depth mechanistic analysis of genotoxicity. Once set up, such an analysis can easily be scaled up to hundreds of samples.