Organoid Volume Quantification


Automated analysis of organoid growth and differentiation is important for toxicity assays, drug screening and quality control protocols.

This solution can be used to quantify the volume of organoids and their lumens if applicable. As a first step, the organoid area and the lumen area are segmented using a trained deep learning network. This segmentation is then applied to z-stack images of many organoids gathered from multi-well plates.

Figure 1: 3D Visualization of an organoid (green) and lumen (magenta)

For each organoid, 3D objects are generated that are used to quantify the organoid and lumen volumes. Finally, the measurements are presented as tables and interactive plots making it easy to understand and interpret the results.

Figure 2: Volume of whole organoids and lumen plotted for a selection of organoids. Based on total volume, organoid growth was assessed. Based on the ratio of lumen per organoid, the differentiation of each individual organoid was assessed.

Deep learning, Apeer, arivis Vision4D, Celldiscoverer7, organoid, growth, differentiation
More information:
Image Source:
Example sample shown here was kindly provided by M. Lutholf. Imaging was performed by Frank Vogler, ZEISS Demo Center Oberkochen.

Contact us!

Feel free to request more information about what we offer to the biotech and pharma industry or ask further questions.
We are here for you.

Get in Touch
Do you want to learn more? Contact us